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Freezing --- Freezing --- Biological preservation --- Biological preservation --- Freeze drying --- Freeze drying --- Refrigerants --- Refrigerants

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lactic acid bacteria --- lactic acid bacteria --- Lactobacillus bulgaricus --- Lactobacillus bulgaricus --- Lactic fermentation --- Lactic fermentation --- Biological preservation --- Biological preservation --- Freeze drying --- Freeze drying

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Oxalis tuberosa --- Oxalis tuberosa --- genetic variation --- genetic variation --- Biological preservation --- Biological preservation --- In vivo experimentation --- In vivo experimentation --- Varieties --- Varieties --- genetic markers --- genetic markers --- Bolivia (Plurinational State of) --- Bolivia (Plurinational State of)

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Beta vulgaris --- Beta vulgaris --- Meristems --- Meristems --- Cell culture --- Cell culture --- Freezing --- Freezing --- Biological preservation --- Biological preservation --- Adaptation --- Adaptation --- Encapsulation --- Encapsulation --- Linoleic acid --- Linoleic acid --- Sucrose. --- Sucrose --- Osmotic pressure --- Osmotic pressure --- Plant tissues --- Plant tissues --- cell structure --- cell structure --- Models --- Models --- Histologie

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A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book’s main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology™ series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

Embryon végétal --- Plant embryos --- Culture d'embryon --- Embryo culture --- Développement embryonnaire --- Embryonic development --- Zygotes --- Conservation biologique --- Biological preservation --- Culture in vitro --- In vitro culture --- Fécondation in vitro --- In vitro fertilization --- Embryogénèse somatique --- Somatic embryogenesis --- Transformation génétique --- genetic transformation --- Plant embryology --- Plant embryology, --- Plants, --- Seeds. --- Plants --- Laboratory manuals. --- Embryology. --- embryology.